Facilitair Medewerker AMOLF & ARCNL

Werkzaamheden
Je voornaamste taken zijn:

• Verricht algemene facilitaire ondersteuning binnen en buiten het gebouw.
• Beheert ruimten en maakt deze klaar voor gebruik.
• Ondersteunt bij interne verhuizingen.
• Verricht kleine onderhoudswerkzaamheden en verhelpt storingen waar mogelijk en/of schakelt na goedkeuring derden in voor het oplossen van storingen, reparaties en onderhoud binnen afgesproken contracten.
• Helpt, indien nodig, bij lichte schoonmaakwerkzaamheden.
• Verricht hand- en spandiensten zoals het ophangen van whiteboards en kantoorinrichting en ondersteunt bij de organisatie van interne evenementen of bijeenkomsten.

Voorafgaande ervaring met de bovengenoemde taken is een pré, maar niet vereist voor sollicitatie, omdat ontbrekende expertise zal worden aangevuld door training op de werkvloer. Het is dan wel belangrijk dat je het leuk vindt om nieuwe taken leren.

Wat wij vragen
De ideale kandidaat voor deze functie:

• Is altijd bereid collega’s te helpen.
• Kan met (eventueel) hulp van ARBO-middelen fysieke taken uitvoeren.
• Beheerst de Nederlandse of Engelse taal. Nederlands is voor ons geen must.
• Heeft affiniteit met gereedschappen en weet hoe deze te gebruiken.
• Kan zelfstandig werken, toont initiatief en is een teamspeler.
• Kan overzicht over meerdere opdrachten tegelijk houden.
• Is bereid opleidingen te volgen.

Het betreft een functie op MBO-3 werk- en denkniveau, maar we willen ook schoolverlaters met werkervaring uitnodigen om te solliciteren.

Hier kom je te werken
De afdeling ondersteunt twee onderzoeksorganisaties; AMOLF en ARCNL. Bij deze organisaties op het Amsterdam Science Park werken in totaal circa 240 onderzoekers en 80 ondersteunende medewerkers. AMOLF initieert en verricht toonaangevend fundamenteel onderzoek aan nieuwe, strategisch belangrijke complexe molecuul- en materiaalsystemen, in samenwerking met de Nederlandse universiteiten en industrie. Zie ook www.amolf.nl.

Het Advanced Research Center for Nanolithography (ARCNL) verricht fundamenteel onderzoek aan de fysica en technologie van nano-lithografie, in het bijzonder voor toepassing in de halfgeleiderindustrie. Het ARCNL is een publiek-private samenwerking tussen ASML en meerdere kennisinstellingen. Zie ook www.arcnl.nl.

Wat wij bieden

• De werksfeer op het instituut wordt in hoge mate bepaald door jonge, enthousiaste, veelal buitenlandse medewerkers. De communicatie is informeel en verloopt via korte lijnen.
• Het betreft een dienstverband voor 40 uren per week van twee jaar met de intentie om aansluitend een vast dienstverband aan te bieden.
• Inschaling vindt plaats in schaal 5, 6, minimaal € 2.767,00 en maximaal € 3.438,00 bruto per maand (afhankelijk van je opleiding en ervaring), te vermeerderen met het vakantiegeld (8%) en een eindejaarsuitkering (8,33%).
• Onze uitstekende secundaire arbeidsvoorwaarden zijn hier te raadplegen. Training on the job en studiefaciliteiten zijn vanzelfsprekend.

Meer informatie?
Voor nadere inlichtingen over de inhoud van de functie kun je je wenden tot Nik Noest via n.noest@amolf.nl

Solliciteren
Lijkt deze baan iets voor jou? Reageer dan via de onderstaande knop!

Online screening kan deel uitmaken van de selectieprocedure.

Diversiteitscode
Wij streven naar een divers personeelsbestand: we willen talent en creativiteit ontwikkelen door mensen met verschillende achtergronden en culturen samen te brengen. We werven en selecteren op basis van competenties en talenten. We moedigen iedereen met de juiste kwalificaties ten zeerste aan om te solliciteren op de vacature, ongeacht leeftijd, geslacht, afkomst, seksuele geaardheid of fysieke vaardigheden.

Acquisitie naar aanleiding van deze advertentie wordt niet op prijs gesteld.

Facilitair Medewerker AMOLF & ARCNL

Work Activities
Als medewerker facilitair ben je altijd op zoek naar een manier om het gebouw en de faciliteiten te verbeteren. Je wordt onderdeel van de facilitaire dienst die valt onder facilities service. Samen met de technische dienst onderhoudt dit team de installaties en de kantooromgeving. We ondersteunen een levendige groep van onderzoekers en support medewerkers om met hen de werkomgeving zo prettig mogelijk te houden.

Het betreft een nieuwe functie in het team waarbij je samen met één collega zorgdraagt voor de representativiteit van AMOLF en ARCNL. Het team bestaat hiernaast nog uit drie technisch facilitair medewerkers die de installaties onderhouden. Het is een platte organisatie waarbij samenwerking met de supportafdelingen en de actieve personeelsvereniging gestimuleerd wordt.

Je voornaamste taken zijn:

• Verricht algemene facilitaire ondersteuning binnen en buiten het gebouw.
• Beheert ruimten en maakt deze klaar voor gebruik.
• Ondersteunt bij interne verhuizingen.
• Verricht kleine onderhoudswerkzaamheden en verhelpt storingen waar mogelijk en/of schakelt na goedkeuring derden in voor het oplossen van storingen, reparaties en onderhoud binnen afgesproken contracten.
• Helpt, indien nodig, bij lichte schoonmaakwerkzaamheden.
• Verricht hand- en spandiensten zoals het ophangen van whiteboards en kantoorinrichting en ondersteunt bij de organisatie van interne evenementen of bijeenkomsten.

Voorafgaande ervaring met de bovengenoemde taken is een pré, maar niet vereist voor sollicitatie, omdat ontbrekende expertise zal worden aangevuld door training op de werkvloer. Het is dan wel belangrijk dat je het leuk vindt om nieuwe taken leren.

Qualifications
De ideale kandidaat voor deze functie:

• Is altijd bereid collega’s te helpen.
• Kan met (eventueel) hulp van ARBO-middelen fysieke taken uitvoeren.
• Beheerst de Nederlandse of Engelse taal. Nederlands is voor ons geen must.
• Heeft affiniteit met gereedschappen en weet hoe deze te gebruiken.
• Kan zelfstandig werken, toont initiatief en is een teamspeler.
• Kan overzicht over meerdere opdrachten tegelijk houden.
• Is bereid opleidingen te volgen.

Het betreft een functie op MBO-3 werk- en denkniveau, maar we willen ook schoolverlaters met werkervaring uitnodigen om te solliciteren.

Work environment
The department supports two research organisations; AMOLF and ARCNL. These institutes are located at the Amsterdam Science Park and currently employs about 240 researchers and 80 support employees.

AMOLF initiate and performs leading fundamental research on the physics of complex forms of matter, and to create new functional materials, in partnership with academia and industry. www.amolf.nl

ARCNL performs fundamental research, focusing on the physics and chemistry involved in current and future key technologies in nanolithography, primarily for the semiconductor industry. ARCNL’s research program are intimately connected with the interests of the industrial partner ASML. www.arcnl.nl

Working conditions
De sfeer op het Instituut wordt in hoge mate bepaald door jonge, enthousiaste medewerkers. De communicatie is informeel en verloopt rechtstreeks met de werknemers. De secundaire arbeidsvoorwaarden zijn uitstekend. Interne training en studiefaciliteiten zijn vanzelfsprekend. Inschaling vindt, afhankelijk van relevante kennis en ervaring, plaats in schaal 5 of 6; maximaal € 3.438,- bruto per maand op basis van een fulltime dienstverband exclusief vakantiegeld (8%) en eindejaarsuitkering (8,33%). De functie is voor 32 – 40 uur. We bieden een dienstverband van twee jaar, met de intentie van aansluitend een vast dienstverband.

More information?
For further information about the position, please contact Nik Noest: n.noest@amolf.nl

Application
You can respond to this vacancy online via the button below.

Online screening may be part of the selection.

Diversity code
AMOLF and ARCNL are highly committed to an inclusive and diverse work environment: we want to develop talent and creativity by bringing together people from different backgrounds and cultures. We recruit and select on the basis of competencies and talents. We strongly encourage anyone with the right qualifications to apply for the vacancy, regardless of age, gender, origin, sexual orientation or physical ability.

Commercial activities in response to this ad are not appreciated.

Postdoc: Single-molecule microscopy of checkpoint therapeutic action on T cells

Work Activities
As postdoc member of the REPRESSIT consortium coordinated the Physics of Cellular Interactions group headed by Dr. Kristina Ganzinger, you will work on developing a new type of cancer therapeutic (checkpoint inhibitor) in a biophysical project right at the interface of biochemistry, biophysics and immunology in an EIC-funded European consortium.

Checkpoint blockage focuses on targeting inhibitory immune receptors with antibodies to prevent their activation by blocking ligand binding. This strategy has revolutionised immunotherapy helping millions of cancer patients with some spectacular results, providing a novel path to potentiate immune responses against tumour cells. Nevertheless, most patients fail to benefit from these antibody-based therapies highlighting critical pitfalls of the current checkpoint blockade approach: one of them is that blocking receptor-ligand interactions is insufficient to shut down inhibitory signalling completely.

By taking up this postdoc position, you will help changing the current ligand-centric “blockade” paradigm and focus on developing molecules that induce dephosphorylation of immune receptors (so-called RIPR molecules) to shut down their signalling entirely, in contrast to currently available therapeutics. Our approach termed REPRESSIT (Regulated phosphatase recruitment ends sustained signaling of inhibitory targets) can completely shut down even tonic inhibitory receptor signaling (Fernandes, R.A. et al. Immune receptor inhibition through enforced phosphatase recruitment. Nature 586, 779–784 (2020)). But how do this phosphase-recruiting molecule need to be designed to optimally shut down signalling?

In close collaboration with the Fernandes group (University of Oxford) and our other REPRESSIT partners, you will analyse the spatiotemporal dynamics of how different designs of RIPR molecules (Receptor Inhibition by Phosphatase Recruitment) modulate T cell receptor signalling both in live cells and model lipid bilayers with reconstituted receptor domains, using single-molecule fluorescence microscopy and single-particle tracking techniques. You will study both receptor organisation and receptor signalling (adaptor protein recruitment). From these experiments, we will understand the molecular basis of RIPR function and determine general biophysical design principles to further improve the potency of RIPR molecules. The performance of promising new RIPR designs we find will then be tested and compared against conventional antibodies preclinical models by our REPRESSIT partners. Ultimately, this project aims at developing and establishing proof-of-concept of the REPRESSIT platform for bringing these molecules to the clinic and patients.

Qualifications
You need to meet the requirements for a doctors-degree (PhD) and must have research experience in a non-Dutch academic environment. We are looking for a (bio)physicist who has already worked with single-molecule fluorescence techniques or an experimental (bio)chemist with strong experience in biophysical techniques and data analysis. Experience with single-molecule fluorescence microscopy or super-resolution microscopy, automated data analysis and coding (Python) is highly desired; knowledge of cell culture and/or protein biochemistry and/or model lipid systems would be an advantage. You should like the idea of working in a collaborative, ambitious and international environment. We ideally look for a candidate who can start this position in March 2025.

Work environment
AMOLF is part of NWO-I and initiate and performs leading fundamental research on the physics of complex forms of matter, and to create new functional materials, in partnership with academia and industry. The institute is located at Amsterdam Science Park and currently employs about 140 researchers and 80 support employees. www.amolf.nl.

Our group – We are a supportive and social research group of about 8 PhD students and postdocs, which work together in small teams on various projects from immunological signalling to signalling in synthetic cells. The collaborative and social atmosphere includes to the other research groups at the AMOLF institute at the Science Park in Amsterdam East. Our group has been making recent technical advances, including a new single-molecule tracking method based on DNA-PAINT that we recently developed (Nat Commun 2021, Nat Commun 2023), new in vitro assays (ACS Syn Biol 2021, ACS Omega 2024), and imaging approaches developed by our lab and others (JACS 2013, Nat Immun 2016, PNAS 2019), all to closely investigate the intricate molecular details of cellular signaling.

https://amolf.nl/research-groups/physics-of-cellular-interactions

Working conditions

• The working atmosphere at the institute is largely determined by young, enthusiastic, mostly foreign employees. Communication is informal and runs through short lines of communication.
• The position is intended as full-time (40 hours / week, 12 months / year) appointment in the service of the Netherlands Foundation of Scientific Research Institutes (NWO-I) for the duration of 2 years.
• Salary is in scale 10 (CAO-OI) which starts at 4.065 Euro’s gross per month, and a range of employment benefits.
• AMOLF assists any new foreign Postdoc with housing and visa applications and compensates their transport costs and furnishing expenses.

More information?
For further information about the position, please contact Kristina Ganzinger: k.ganzinger@amolf.nl or Raquel Martinez Gonzalez: R.MartinezGonzalez@amolf.nl.

Application
You can respond to this vacancy online via the button below.

Online screening may be part of the selection.

Diversity code
AMOLF is highly committed to an inclusive and diverse work environment: we want to develop talent and creativity by bringing together people from different backgrounds and cultures. We recruit and select on the basis of competencies and talents. We strongly encourage anyone with the right qualifications to apply for the vacancy, regardless of age, gender, origin, sexual orientation or physical ability.

AMOLF has won the NNV Diversity Award 2022, which is awarded every two years by the Netherlands Physical Society for demonstrating the most successful implementation of equality, diversity and inclusion (EDI).

Commercial activities in response to this ad are not appreciated.

Postdoc: Single-molecule microscopy of checkpoint therapeutic action on T cells

Work Activities
As postdoc member of the REPRESSIT consortium coordinated the Physics of Cellular Interactions group headed by Dr. Kristina Ganzinger, you will work on developing a new type of cancer therapeutic (checkpoint inhibitor) in a biophysical project right at the interface of biochemistry, biophysics and immunology in an EIC-funded European consortium.

Checkpoint blockage focuses on targeting inhibitory immune receptors with antibodies to prevent their activation by blocking ligand binding. This strategy has revolutionised immunotherapy helping millions of cancer patients with some spectacular results, providing a novel path to potentiate immune responses against tumour cells. Nevertheless, most patients fail to benefit from these antibody-based therapies highlighting critical pitfalls of the current checkpoint blockade approach: one of them is that blocking receptor-ligand interactions is insufficient to shut down inhibitory signalling completely.

By taking up this postdoc position, you will help changing the current ligand-centric “blockade” paradigm and focus on developing molecules that induce dephosphorylation of immune receptors (so-called RIPR molecules) to shut down their signalling entirely, in contrast to currently available therapeutics. Our approach termed REPRESSIT (Regulated phosphatase recruitment ends sustained signaling of inhibitory targets) can completely shut down even tonic inhibitory receptor signaling (Fernandes, R.A. et al. Immune receptor inhibition through enforced phosphatase recruitment. Nature 586, 779–784 (2020)). But how do this phosphase-recruiting molecule need to be designed to optimally shut down signalling?

In close collaboration with the Fernandes group (University of Oxford) and our other REPRESSIT partners, you will analyse the spatiotemporal dynamics of how different designs of RIPR molecules (Receptor Inhibition by Phosphatase Recruitment) modulate T cell receptor signalling both in live cells and model lipid bilayers with reconstituted receptor domains, using single-molecule fluorescence microscopy and single-particle tracking techniques. You will study both receptor organisation and receptor signalling (adaptor protein recruitment). From these experiments, we will understand the molecular basis of RIPR function and determine general biophysical design principles to further improve the potency of RIPR molecules. The performance of promising new RIPR designs we find will then be tested and compared against conventional antibodies preclinical models by our REPRESSIT partners. Ultimately, this project aims at developing and establishing proof-of-concept of the REPRESSIT platform for bringing these molecules to the clinic and patients.

Qualifications
You need to meet the requirements for a doctors-degree (PhD) and must have research experience in a non-Dutch academic environment. We are looking for a (bio)physicist who has already worked with single-molecule fluorescence techniques or an experimental (bio)chemist with strong experience in biophysical techniques and data analysis. Experience with single-molecule fluorescence microscopy or super-resolution microscopy, automated data analysis and coding (Python) is highly desired; knowledge of cell culture and/or protein biochemistry and/or model lipid systems would be an advantage. You should like the idea of working in a collaborative, ambitious and international environment. We ideally look for a candidate who can start this position in March 2025.

Work environment
AMOLF is part of NWO-I and initiate and performs leading fundamental research on the physics of complex forms of matter, and to create new functional materials, in partnership with academia and industry. The institute is located at Amsterdam Science Park and currently employs about 140 researchers and 80 support employees. www.amolf.nl.

Our group – We are a supportive and social research group of about 8 PhD students and postdocs, which work together in small teams on various projects from immunological signalling to signalling in synthetic cells. The collaborative and social atmosphere includes to the other research groups at the AMOLF institute at the Science Park in Amsterdam East. Our group has been making recent technical advances, including a new single-molecule tracking method based on DNA-PAINT that we recently developed (Nat Commun 2021, Nat Commun 2023), new in vitro assays (ACS Syn Biol 2021, ACS Omega 2024), and imaging approaches developed by our lab and others (JACS 2013, Nat Immun 2016, PNAS 2019), all to closely investigate the intricate molecular details of cellular signaling.

https://amolf.nl/research-groups/physics-of-cellular-interactions

Working conditions

• The working atmosphere at the institute is largely determined by young, enthusiastic, mostly foreign employees. Communication is informal and runs through short lines of communication.
• The position is intended as full-time (40 hours / week, 12 months / year) appointment in the service of the Netherlands Foundation of Scientific Research Institutes (NWO-I) for the duration of 2 years.
• Salary is in scale 10 (CAO-OI) which starts at 4.065 Euro’s gross per month, and a range of employment benefits.
• AMOLF assists any new foreign Postdoc with housing and visa applications and compensates their transport costs and furnishing expenses.

More information?
For further information about the position, please contact Kristina Ganzinger: k.ganzinger@amolf.nl or Raquel Martinez Gonzalez: R.MartinezGonzalez@amolf.nl.

Application
You can respond to this vacancy online via the button below.

Online screening may be part of the selection.

Diversity code
AMOLF is highly committed to an inclusive and diverse work environment: we want to develop talent and creativity by bringing together people from different backgrounds and cultures. We recruit and select on the basis of competencies and talents. We strongly encourage anyone with the right qualifications to apply for the vacancy, regardless of age, gender, origin, sexual orientation or physical ability.

AMOLF has won the NNV Diversity Award 2022, which is awarded every two years by the Netherlands Physical Society for demonstrating the most successful implementation of equality, diversity and inclusion (EDI).

Commercial activities in response to this ad are not appreciated.

Postdoc: The real-time dynamics of single ribosomes

Work Activities
Summary - In this project, you will perform innovative (bio)physics experiments to elucidate the activity of individual ribosomes. The key methodologies are optical tweezers and single-molecule fluorescence. Working in the Tans biophysics lab @ AMOLF Amsterdam, you will visualize the real-time translation activity of ribosomes, how multiple ribosomes work together, detect the folding of individual polypeptide chains, and show how chaperones guide this process. You will work in an international team, with other groups that are specialized in RNA sequencing and cryo-EM techniques. The aim is to elucidate the fundamental mechanisms that underlie the correct and erroneous production of new proteins.

Questions - According to textbooks, ribosomes work individually to produce proteins. However, recent insights from our lab and others show multiple ribosomes cooperate as they translate mRNA. This cooperation may well be essential to produce multi-protein complexes faithfully. In our group, we aim to elucidate the highly diverse and broad implications. Questions include: Do ribosomes synchronize their translation activity in real-time, like a sequence of cars in traffic? Are mRNA secondary structures and/or direct interactions between ribosomes involved? How do the two (or more) protein chains synthesized by these multiple ribosomes fold together to form protein complexes? How many ribosomes cooperate together? Can we see cooperation between ribosomes that translate different RNA messages? Given the novelty of these fundamental questions in this rapidly expanding field, you will have a unique chance to address them first.

Approach - You will directly follow the unknown dynamics of ribosome translation, protein folding and assembly, and chaperone guidance. This is enabled using optical tweezers combined with single-molecule fluorescence, which detect changes in individual molecules at nanometer and millisecond resolution. Direct collaboration with our partners provides complementary genome-wide in-vivo data. This first look may reveal a host of unexpected phenomena. You will develop new experimental schemes, use cutting-edge single-molecule fluorescence and manipulation methods, adapt existing biochemical protocols, analyze the complex temporal data, formulation of new models, and explain your findings in high-level scientific papers.

International team - You will be part of a collaboration with leading groups at Heidelberg University and the ETH in Zurich, which use novel sequencing and cryo-EM methods. By working within this motivated group of young scientists, you will obtain a unique training, understanding and skill set in this expanding field. By integrating these approaches, you will provide insights of unprecedented detail, spanning from the cellular to the atomic level, from in vivo to in vitro, from genome-wide patterns to molecular mechanisms, and from bacteria to human cells.

Image left show ribosome (blue, orange) that synthesized protein chain (yellow) by translating the RNA message (red). Image right shows dynamic ribosome interactions.

Qualifications
We have a number of positions available (PhD and PD). We are looking for outstanding experimental physicists or chemists with an interest in single-molecule techniques, ambition to make breakthrough findings, programming skills to handle complex data, and who thrive in a diverse, collaborative, and supportive environment. Excellent verbal and written English skills are essential.

Work environment
AMOLF is a part of NWO-I and initiate and performs leading fundamental research on the physics of complex forms of matter, and to create new functional materials, in partnership with academia and industry. The institute is located at Amsterdam Science Park and currently employs about 140 researchers and 80 support employees. www.amolf.nl

Our group - We form a lively and close-knit research group of about 10 PhD students and postdocs, which work together in small teams on various projects in a highly supportive and social atmosphere that extends to the other research groups at the AMOLF institute, which is housed in a modern building in the east of Amsterdam. The Tans group at the AMOLF institute Amsterdam has been at the forefront of studying chaperone-guided protein folding using optical tweezers. This technique allows one to mechanically manipulate individual proteins, and hence follow the movements and folding steps as they fold. We have shown striking sequences of molecular events that underlie chaperone functions, which are invisible with other methods.

https://www.sandertanslab.nl

https://amolf.nl/research-groups/biophysics

Working conditions

• The working atmosphere at the institute is largely determined by young, enthusiastic, mostly foreign employees. Communication is informal and runs through short lines of communication.
• The position is intended as full-time (40 hours / week, 12 months / year) appointment in the service of the Netherlands Foundation of Scientific Research Institutes (NWO-I) for the duration of four years
• Funding is available to attend regularly international conferences.
• AMOLF assists any new foreign researcher with housing and visa applications and compensates their transport costs and furnishing expenses.

More information?
For further information about the position, please contact

Prof. dr. ir. Sander Tans
tans@amolf.nl
https://www.sandertanslab.nl
Phone: +31-(0)20-754 7100

Application
You can respond to this vacancy online via the button below.

Please annex your:

• Resume;
• Motivation on why you want to join the group (max. 1 page).

It is important to us to know why you want to join our team. Hence, we will only consider your application if it contains your motivation letter.

Applications will be evaluated on a rolling basis and as soon as an excellent match is made, the position will be filled.

Online screening may be part of the selection.

Diversity code
AMOLF is highly committed to an inclusive and diverse work environment: we want to develop talent and creativity by bringing together people from different backgrounds and cultures. We recruit and select on the basis of competencies and talents. We strongly encourage anyone with the right qualifications to apply for the vacancy, regardless of age, gender, origin, sexual orientation or physical ability.

AMOLF has won the NNV Diversity Award 2022, which is awarded every two years by the Netherlands Physical Society for demonstrating the most successful implementation of equality, diversity and inclusion (EDI).

Commercial activities in response to this ad are not appreciated.